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2 edition of Measuring bacterial glucose mineralization in an acid-stressed lake found in the catalog.

Measuring bacterial glucose mineralization in an acid-stressed lake

John M. McDonald

Measuring bacterial glucose mineralization in an acid-stressed lake

by John M. McDonald

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  • 36 Currently reading

Published by Laurentian University in Sudbury, Ont .
Written in English


Edition Notes

StatementJohn M. McDonald.
The Physical Object
Pagination42 l. :
Number of Pages42
ID Numbers
Open LibraryOL22161819M

Dissolved organic carbon (DOC) is the fraction of total organic carbon operationally defined as that which can pass through a filter size that typically ranges in size from and micrometers. The fraction . A cid from Glucose. What is the purpose of the test? The purpose is to see if the microbe can ferment the carbohydrate (sugar) glucose (also known as dextrose) as a carbon source. How is glucose (dextrose) fermentation determined? If glucose .

Five oligotrophic lakes in southern Sweden were sampled in late autumn. Phosphate-P and glucose-C alone or in combination ( and mg L −1, respectively) was added to µm filtered lake water and incubated in darkness at 20°C. Additions of glucose (C) and phosphorus (P) alone did not lead to changes in the rates of bacterial. list of recent articico, books, and documents available in the Robert "Measuring effects of air pollution stress on forest productivity: perspectives, problems, and approaches." Choquet, C.G. "Bacterial glucose mineralization in acid-stressed. lake .

The availability of essential nutrients, such as nitrogen (N) and phosphorus (P), can feedback on soil carbon (C) and the soil microbial biomass. Natural cycles can be supplemented by agricultural . For measuring respiration the microcosm vials selected for measurement, together with five sterile controls with normal CaC[3] and glucose additions, were placed in mL jars. The jars were .


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Measuring bacterial glucose mineralization in an acid-stressed lake by John M. McDonald Download PDF EPUB FB2

Instead, neutrophilic bacterial populations tolerant to lower pH's were present in such lakes. It was the purpose of this research to quantitate, seasonally, the mineralization response of the benthic microbiota of an extremely acid-stressed lake, as well as to measure Cited by: 1.

A method is described for measuring the mineralization of an organic solute (14 C-glucose) by the heterotrophic indigenous bacteria in lake sediments.

Since there is no suitable procedure for the determination of in situ microbial Cited by: This paper concerns bacterial glucose mineralization in Silver Lake sampled during the summer; specifically, whether or not it occurs, and whether or not the “heterotrophic activity method” can be used to measure it, in this acidified by: 7.

This paper concerns the kinetic approach to measuring glucose mineralization by planktonic bacteria in Whitson Lake, a moderately acidic lake. For all three samplings, plots of the amount of glucose mineralized vs time of incubation were linear at both high and low concentrations of added by: 4.

A method is described for the incubation of undisturbed sediment cores under in situ conditions with the addition of low concentrations of 14C-glucose.

Data are presented for respiration, gross uptake and actual uptake rate of glucose by bacteria in sandy, wave-washed beaches of the Baltic Sea.

On the average, the bacteria respired 8% of the total glucose Cited by: We have already reported that the method is useful in simulated acid rain studies employing samples from a circumneutral lake [2], but that it is unreliable in studying glucose mineralization activity in the open water of an extremely acid-stressed lake [3] and even in the open water of a moderately acid-stressed lake.

For the acid-stressed Silver Lake, glucose mineralization was negligible at the in situ pH () and at the experimental pH's ( and ) as well.

The application of this method in the study of acid. Data indicated that bacterial populations and densities were nearly an order of magnitude less in acid stressed waters than in non-acid stressed waters. Nitrifying bacteria and some sulfur cycle. A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text.

Glucose mineralization rates were measured at 4°C and 18°C in sediment samples taken during ice-cover from an oligotrophic lake and a eutrophic lake and were found to fit Michaelis-Menten kinetics.

Effect of Temperature on Heterotrophic Glucose Uptake, Mineralization, and Turnover Rates in Lake Sediments Article (PDF Available) in Applied and Environmental Microbiology 43(1) February.

Specific activity of aquatic bacteria, which indicates average heterotrophic activity per bacterial cell, was determined asV max per bacterium and turnover rate per bacterium for glucose mineralization at.

Pseudomonas saccharophila P15 was isolated from soil contaminated with polycyclic aromatic hydrocarbons (PAH) and previously was reported to degrade a variety of low- and high-molecular.

Each incubation device was destructively sampled at day intervals for days and net N mineralization was detd. by measuring the amt. of inorg. N that accumulated in the soil or soil plus. Suspended bacteria in calcareous and acid headstreams: abundance, heterotrophic activity and downstream change Article in Freshwater Biology 16(5) - May with 9 Reads.

Abstract. The decomposition of organic matter in aquatic ecosystems by microorganisms generally involves two processes: (1) the hydrolytic degradation of high molecular weight organic polymers into compounds of low molecular weight, such as glucose, cellobiose, and amino acids; and (2) the nonhydrolytic oxidative mineralization.

The process of transformation of dissolved organic matter into bacterial biomass is one of the basic metabolic turnover patterns in a lake system. The biomass production by heterotrophic bacteria can. Glucose uptake was monitored on a seasonal basis, using [ H]glucose and undisturbed cores collected from an intertidal mud flat.

The fate of glucose carbon, including the formation of CO 2 and biomass, was assayed by using undisturbed cores and [U- 14 C]glucose; the production of short-chain fatty acids was monitored with [U- 14 C]glucose. The glucose uptake kinetics of some marine bacteria.

Can J Microbiol. Oct; 12 (5)– Harrison MJ, Wright RT, Morita RY. Method for measuring mineralization in lake sediments. Appl Microbiol. Apr; 21 (4)– [PMC free article] Tison DL, Pope DH.

Effect of temperature on mineralization by heterotrophic bacteria. Bacteria played a major role in glucose mineralization in both the rhizosphere and rhizoplane. These results indicate that the bacteria may play a greater role in glucose mineralization processes in the rhizosphere and rhizoplane zones of a semiarid grassland.

Glucose represented an energy source used by a broad variety of bacteria, whereas fewer soil species were expected to use xylene. The metal amendments were chosen to inhibit the acute rate of organic mineralization by either 50% or 90%, and lower mineralization.

FIGURE 5. Laboratory study of Michaelis–Menten kinetics from the incorporation of glucose at a variety of concentrations by a lake bacteria culture (low K m, low V max) and an algal culture (Chlamydomonas sp., high K m, high V max).

K m of bacteria. For example, if bacteria used glucose for maximum possible enzyme production (see equation for complete glucose anabolism in Herbert, ), the RQ would only be ca units higher .